ABSTRACT
Objective To study the chemical constituents of the stems and leaves of Clausena hainanensis. Methods The chemical constituents of C. hainanensis were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies, and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as comparisons with the data reported in literature. Results Eighteen compounds were isolated from the 90% ethanol extract of the stems and leaves of C. hainanensis, which were identified as mukonine (1), methyl carbazole-3-carboxylate (2), lansine (3), murrayanine (4), 3-formylcarbazole (5), 3-formyl-6-methoxycarbazole (6), lansamide 4 (7), 4-methoxy-N-methyl-2-quinolone (8), (E)-N- (4-methoxyphenethyl)-2-methylbut-2-enamide (9), (E)-N-methyl cinnamon amide (10), N-(2-hydroxy-2-phenylethyl) cinnamamide (11), N-benzoyltyramine (12), aurantiamide (13), N-methyl-2-pyrolidinone (14), coumaric acid (15), 6,8-dimethoxy-4,5-dimethyl-3- methyleneisochromanl-one (16), 8-methoxypsoralen (17), and isololiolide (18). Conclusion This is the first time reporting the chemical constituents from C. hainanensis, all compounds are isolated from C. hainanensis for the first time, and compounds 12-16 are isolated from the genus Clausena for the first time.
ABSTRACT
Objective: To study the chemical constituents from the roots of Clausena excavata (Rutaceae). Methods: The compounds were isolated and purified by means of column chromatographies including silica gel, RP-18, and HPLC. Compound structures isolated were determined on the basis of spectroscopic data. Results: Thirteen carbazole alkaloids clausine K (1), clausine E (2), 3-formyl-7-hydroxycarbazole (3), clausine H (4), clausine M (5), mukona (6), O-demethylmurrayanine (7), clausine O (8), 3-formylcarbazole (9), 3-formyl-6-methoxy carbazole alkaloid (10), clausine C (11), 2-methoxy-3-carboxylic acid-carbazole alkaloid (12), and clausine Z (13) were isolated from the roots of C. excavata. Conclusion: Compounds 9,10, and 12 are isolated from this plant for the first time. Compounds 1,2,7,8, and 10 are tested for their cytotoxicities against A549, Hela, and BGC-823 cancer cell lines. The results show that they exhibit the cytotoxicity against A549, Hela, and BGC-823 with the IC50 values of 8.53-19.87 μg/mL.